planococcus gram stain

Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. The oxygen class determined by the fluid thioglycolate test and the positive results of the Oxidase and Catalase test are both consistent with the oxygenic environment that the microbe was isolated from. This app provided sample information such as the total number of reads that were used for classification and the percent of reads that were classified. Based on multiple Isolation and characterization of a novel hydrocarbon‐degrading, Gram‐positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. Head The identification of this microbe would add to the depth of knowledge of what bacteria are and can be found on decomposing wood in the sub-artic. The Isolate appeared to be a pure culture by the third quadratic streak; a fourth was performed for certainty. Isolated from sub-artic decomposing wood. Colony morphology including color, size, margin and elevation was observed from growth on the TSA agar. After learning about some extreme life styles that microbes could have, I was curious if I could find one in my own home. BaseSpace has many apps that provide different information about the genomic data. The results of the SPAdes Genome Assembler were above required values; # contigs >=1000 bp should be in the hundreds, total length should be at least a million bp and the longest contig should be at least 100000 bp. 5. Your email address will not be published. BLAST provided many species level classifications with similar query cover and percent identity. to the Genus. Planococcus antarcticus and Planococcus psychrophilus spp. Colonies are round and yellow in color. Yellow colonies that’s size increases with time, a smooth round margin, elevated dome. 1 micrometer diameter, coccus, cells divide linearly. The results did not meet these thresholds, and this is the reason that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected. BLAST suggested Genus and species with associated query cover percentage and identity percentage. Taxonomic studies were performed on a novel carotenoid-producing strain, designated AJ003T, isolated from faeces of Antarctic penguins. Phylogenetic and signature nucleotides analyses revealed that strain Y42T belongs to the genus Planococcus. The DNA was then sent to the UAF’s DNA Core Lab sequenced using the Illumina MiSeq DNA sequencer which utilized the next-generation sequencing methodology. The next app that was used was the Kraken Metagenomics, which provided taxonomic assignment. The isolate was found to be susceptible to all antibiotics tested with the disk diffusion test. Fackrell, H. (n.d.). Karlsruhe 1:235-238, p 1781 Genera related Member of the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection. The cells inoculated onto Eosin Methylene Blue (EMB) and MacConkey Agar (MAC) plates. A hallmark of the genus Planococcus is that they are usually halo-tolerant gram-positive bacteria that frequently inhabit Antartica4. Once identified, I could then research the species and compare it’s physiology and genomic data to current literature to confirm the taxonomic assignment. Pigments may be produced by some species; ranging from cream, pale yellow to light pink. but poor growth less than 1% or more than 15% NaCl the following information is not yet verified Small Gram negative coccobacilli with O-shaped morphology Culture characteristics. Planococcaceae, a family within the order Bacillales, embraces 14 genera: Bhargavaea, Caryophanon, Chryseomicrobium, Filibacter, Jeotgalibacillus, Kurthia, Paenisporosarcina, Planococcus, Planomicrobium, Rummeliibacillus, Solibacillus, Sporosarcina, Ureibacillus, and Viridibacillus.Members of the family are Gram-variable, spore forming or nonspore forming, and motile … It forms orange colonies and grows at NaCl concentrations of 2-10 % (w/v) (optimum, 4 % w/v), at 4-50 °C (optimum, 30 °C) and at pH 6.0-10.0 (optimum, pH 7.0). Planococcus citreus is Gram stain positive and aerobic. Table 2: Antibiotic Susceptibility Results. Cell lysis was accomplished through adding sodium dodecyl sulfate (SDS), which is a surfactant that chemically weakened the cell walls, and the use of PowerBeads and the centrifuge to mechanically break the cell walls. The API 20e tests had negative results for all for the 21 tests: Image 3. Considering the limit of species identified to this date, there is the potential that this microbe could be a new species. Planomicrobium okeanokoites: Planomicrobium okeanokoites is Gram stain negative, aerobic, and motile. Gemella Each of those colonies represented a microbe that had the physiological capability of surviving those conditions, and I was interested in observing them in the lab. The firewood pile was slowly decomposing, and it is likely that the microbe that was isolated from it was a part of the community that was utilizing the wood as an energy source. A suspension of the microbe in broth was tested simultaneously in all 21 of the tests included in the API 20E test strip. The Microbes transfer to the agar slant was successful: Image 1, and was successfully re-cultured from the slant when necessary. Decomposing communities frequently have a mix of fungi and bacteria. The species classification with the highest query cover is poorly documented in literature. One such mechanism is the use of Catalase to catalyze the transformation of the reactive species hydrogen peroxide into O2. Microscopically, it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter (Fig. nov., a carotenoid-producing species isolated from stools of Antarctic penguins. Arb. There are some qualities that are consistent between my sample and P. donghaensis, they are both, gram-positive, aerobic, coccus and oxidase positive 1. Micrococcus sedentarius 9. Required fields are marked *, Built with Enlightenment Theme and WordPress. Cells of strain AJ003T were aerobic, Gram-stain-positive, cocci-shaped and orange. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Colony morphology on the TSA agar revealed a yellow colony that’s size increased with time, a smooth round margin and the colony was an elevated domed. The TSA medium provided a suitable environment for a variety of bacteria and a few molds. The negative results and lack of growth in the API 20e, MAC, and EMB are all consistent with the microbe’s gram-positive cell wall. The percent of contigs read needed to be greater than 80% and the percent of those reads that needed to agree upon a classification to trust that classification to the genus level was 80% and to the genus level was 60%. Colony morphology for these organisms vary depending upon the type of media on which they are cultivated. The microbe was susceptible to all eight antibiotics tested. The gram stain was performed to assess the thickness of the peptidoglycan wall. Craters may form around the colonies on some media. Stomatococcus coccus-shaped gram-positive of the genus Planococcus are that the species are Gram-positive, aerobic, non-spore-forming and have cell shapes that include cocci, short rods, or rods (Gan, Zhang, Zhang, et al., 2018). Several other species had similar query cover, including Planococcus antarticus: Image 7. This extremophile is characterized as both halophilic and psychrophilic, thriving in an environment of high salinity as well as an extremely low tem… Micrococcus kristinae 4. The contig.fasta file from the SPAdes Genome Assembly produced by Illumina’s BaseSpace was analyzed by BLAST. The gram stain was performed to assess the thickness of the peptidoglycan wall. Many of the morphological and physiological analyses made on the isolate are not held by every species in the Planoccocus genus. radioresistant cocci called Deinococcus Sterile water was used to moisten the wood and a sterile swab was rolled along the wood’s surface. Image 6 To 20 Classification Results by Taxonomic Level from BaseSpace. The quadratic streak was used 4 times to ensure a pure culture. Image 5: Krona Classification Chart from BaseSpace displays the large portion of unclassified reads. After a two-day waiting period while the microbe was given the opportunity to develop where it could, the diameter of prohibited growth was measured and compared to standard antibiotic specific diameters and then classified as either susceptible, intermediate or resistant. One representative of each from gram stain positive and gram stain negative bacteria was selected to further assess the MIC. Planococcus. Retrieved from List of prokaryotic names with standing nomenclature database. The gram-stain was repeated three times before a successful stain was accomplished. To thrive in this community it would be helpful to have mechanisms to process radical oxygen species. Micrococcus luteus 5. The bacterium is Gram-positive and possesses all the characteristics of the genus Planococcus. Of this information, only the number of contigs that were greater than 1000 base pairs long [# contigs (>=1000bp)], The total length of the assembled genome, the longest contig and the guanine and cytosine percentage in the genome (GC%) were observed. Micrococcus, At this time the culture was transferred into an agar slant. By Ethan Hanson Classification: Kingdom: Bacteria Phylum: Firmicutes Class: Bacilli Order: Bacillaes Family:Planococcaceae Genus: Planococcus Species: Planococcus halocryophilus Planococcus halocryophilus is an aerobic, gram-positive bacterium that is found in arctic permafrost. The morphological characteristics that are consistent with the isolate and across the genus are: gram-positive membranes, cocci cell shape, colonies are yellow orange in color, catalase positive and an aerobic oxygen class5. (2016). “Interactions between saprotrophic basidiomycetes and bacteria,’ in. The SPAdes Genome Assembler provided the overall result of the genomes assembly. I was unable to grow any microbes from the wood stove but the growth from the previously frozen wood was abundant. The cell walls of Gram positive bacteria differ structurally from the cell … Inst. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. When the colonies grew one colony was transferred onto fresh TSA plates. Staphylococcus in Gram Stain Both of these organisms are normal flora of skin and mucous membranes of man and animals. Considering that the API 20e test is designed for enteric gram negative microbes these results are confirmation that the microbe is gram-positive. BLAST had a different database and could possibly have the genome of the isolates species if base space did not. the following information is not yet verified. Phylogenetic composition and properties of bacteria coexisting with the fungus, de Boer W., van der Wal A. BLAST classified the organism as Planoccus donghaensis with a query cover of 77% and a 78% identity. Obligate Aerobic Colonies after 24 hours, gray white, convex and 0.5-1 mm, after 48-72 hours, mucoid to very mucoid, no pigment, non hemolytic The assigned taxonomy all the way to the species level was displayed in both the Krona classification chart and the Top 20 Classification Result by taxonomic level. Halotolerant will grow in nutrient agar have atypical Gram positive cell walls. To control growth rates the plates were grown in incubators for 2-4 days at 37 degrees Celsius then moved to a refrigerator to ensure colonies did not grow large enough to touch. MAC plates are also selective for Gram-negative microbes and differentiates between lactose fermenters (agar turns pink) and non-lactose-fermenters (agar turns colorless). The purity of the isolate was confirmed with the microscopic observation of a mono-culture. The gram-stain was repeated three times before a successful stain was accomplished. The Inhibition Zones exceed the antibiotic specific threshold diameter for susceptibility see table 2 for details. 1a). Considering the environment in which it was found, there were a few expected results: such as the microbe’s oxygen class and catalase test results. Jeong-Hwa C, Wan-Taek I, Qing-Mei L, Jae-Soo Y, Jae-Ho S, Sung-Keun R, Dong-Hyun R. Reddy G, Prakash J, Vairamani M, Prabhakar S, Matsumoto G. Shivaji S. Tuncer, I. Planococcus citri, commonly known as the citrus mealybug, is a species of mealybugs native to Asia.It has been introduced to the rest of the world, including Europe, the Americas, and Oceania, as an agricultural pest.It is associated with citrus, but it attacks a … Planococcus sp. compare with Marinococcus, Migula W. (1894) Uber BaseSpace’s SPAdes Gemone Assembler analyzed 128 contigs over 1000 base pairs (bp) long, a total length of over 3.7 million bp, the longest contig  was 196 thousand bp, and the GC% was 44.4%. The susceptibility of the isolate to 8 different antibiotics was tested using the Kirby-Bauer Method agar and the disk diffusion test per the protocol of Lab 9. They are prokaryotic in nature meaning they are the earliest forms of the cell without a nucleus.. Plesiomonas shigelloides Micrococcus varians The catalase test produced bubbles and the oxidase test produced a blue color change on the strip indicating that the microbe was positive in both tests. A Gram-staining-positive and aerobic coccus with the ability to degrade petroleum bacterium, designated Y42T, was isolated from the Lenghu oil field located in the northern margin of the Qaidam Basin. These tests reveal nothing more than a conformation that it is indeed a gram-positive microbe. These tests proved that the microbe could thrive in an oxygenated as well as an anoxic environment, that it contained cytochrome c oxidase which is a part of the electron transport chain found in microbes that utilize oxygen, and that it contained the catalase mechanism for dealing with oxidative damage, respectively. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. Micrococcus roseus 8. These results reveal that the cells contained both cytochrome c-oxidase and the catalase enzyme respectively. Planococcus sp. While the cells were prepped in slides some characteristics were observed with the microscope, such as cell alignment shape and length. Sarcina, Catalase positive:Deinococcus,Marinococcus haemolysans, Lactococcus Leuconostoc, mucilaginsos, Streptococcus, Trichococcus flocculiformis, Vagococcus, anaerobes Coprococcus, Peptococcus niger , Peptostreptococcus, Ruminococcus, Melissococcus An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. The literature on the Planococcus species like donghaensis, kocurii, halocryophilus and antarticus often notes how closely related the species are and how further analysis like G-C content, fatty acid strains, DNA-DNA hybridization etc1,2 are needed to differentiate the species. Micrococcus agilis 2. Some of the notable differences between Micrococcus and Staphylococcus are: Bacteria Bacillus megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin as histamin. These communities emit extracellular enzymes to decay the wood, this results in an environment with high acidity and enzymes that are producing free radical oxygen species7. The cells were measured to be 1 micrometer in diameter. The analysis of the DNA with BaseSpace revealed unreliable data. Additionally the isolate underwent the thioglycolate test- for oxygen class, oxidase test- for the presence of cytochrome c-oxidase, and lastly the catalase test- for the presence of catalase enzyme. Throughout the course of this study I isolated one colony and performed many physiological and genomic tests to identify what species my isolate was. Antibiotic resistance of bacterial isolates from sediments of eastern Mediterranean Sea in association with environmental parameters. I sampled two places, a piece of wood from my fire wood stack (which is exposed to the extreme cold temperatures of Alaskan winters) and the ashes from my stove. Euzeby, J. P., & Parte, A. C. (2017, April 1). 16S rRNA gene sequence similarity of strain SCU63 T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %. The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). It was identified in 1894, and was only approved in 19806. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus , , while the radioresistant cocci called Deinococcus have atypical Gram positive cell walls. A series of patented solutions C1-4 were added following the protocol to purify and remove inhibitors and proteins until all that remained was a pure solution of DNA. ein neues System der Bakterien. Retrieved April 11, 2017, from Uwindsor.ca website: https://web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published. Dna was extracted and purified from the isolates cells using protocol from lab 5. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. It is urease and catalase positive. The general features of Planococcus sp. Bacterium Planococcus halocryophilus that is able to thrive at minus 15 degrees Celsius offers clues about microbial life on both Mars and Enceladus. In general, the colonies appear as white, smooth colonies. 1. Firmicutes / “Bacilli” / Bacillales / Planococcaceae / Planococcus Cells coccoid , 1.0–1.2 µm in diameter, occurring singly, in pairs, in groups of three, or as tetrads or clumps of cells. The isolate failed to grow on EMB or MAC plates, considering that both plates are selective for gram negative, these results confirm the results of the gram positive test: Image 4. A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63 T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. The bacterium was found to be aerobic and Gram-stain positive. The APP provides an extensive list of regions that were specifically identified as the above elements. The genotypic test results for this microbe were vague, the results of BaseSpace had a low number of reads that could be classified and of those reads there was little consensus. Gram Positive Bacteria. Stomatococcus Of the few documents that relate to Planococcus donhaensis, there is one that notes the origin of the sample, the South Korean Sea. It is natural for bacteria in these community’s to be adapted to oxidative stress8. Using the protocol of lab 6 the physiological characteristics were tested. Meanwhile, most of the Planococcus bacteria have showed the ability to withstand heavy metals, produce surfactants and adapt to cold and/or saline environments [12–14]. Micrococcus lylae 6. The isolates holotolerance was not tested but the similar cellular membrane composition and habitat conditions indicate that this genus is likely correct. The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). This is consistent with a study that found, to the level of the genus, that Planococcus’ were susceptible to all antibiotics tested3. Genus planococcus. Overnight grown pathogenic bacterial strains of 1 × 10 6 CFU/mL were treated with known concentration of biosurfactants and incubated at … An orange pigmented bacterium, S1, was isolated from a cyanobacterial mat sample collected in the vicinity of Schirmacher Oasis, Maitri, the Indian station, inAntarctica. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus, The multiple sequence alignments of 16S rRNA and housekeeping genes showed that … The genus Planococcus was originally proposed by Migula [1] to accommodate Gram-stain-positive, aerobic and motile cocci of the family Planococcaceae in the phylum Firmicutes. They may be domed, shining, mucoid-type colonies with radial striations or may appear smooth with entire edges. mucilaginsosus. The Prokka Genome Annotation app identified 64 tRNAs, 0 rRNAs, 1 CRISPR, and 3739 CDS. Planococcus faecalis sp. Gram stain. pluton, Pediococcus, Saccharococcus thermophilus, Staphylococcus, Micrococcaceae are not a coherent group. Catalase positive:Deinococcus,Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not a coherent group. Planococcus, Staphylococcus,, Micrococcus halobius 3. Using Illumina’s BaseSpace dashboard to handle the data, the isolate’s genome was analyzed and compared to an existing database to search for similar sequences of DNA. Gram‐positive to Gram‐variable. The first publication of a species under the genus Planococcus was P. citreus. Both sources have specific classification percentages that confer certainty. A gram-positive (gram-variable), beaded organism with delicate branching was recovered from the sputum of a 20-year-old patient with leukemia. The sample was streaked using the quadratic method to grow pure isolated colonies onto a tryptic soy agar (TSA) plate. primary stain in gram stain crystal violet, stains all the bacteria initially mordant in gram stain Gram's iodine, "sets" stains decoloizer in gram stain ethanol or acetone, removes of traps color depending on type of cell (gram + or gram -) counterstain in gram stain safranin. PAMC21323 is a gram (+), motile, psy-chrotrophic bacteria, which can grow over a broad temperature range (5–40 °C). Image 5 and 6 are from the Kraken app. masc. The specimen produced orange, glabrous, waxy colonies on Middlebrook's agar that showed partial acid-fast staining with the modified Kinyoun stain. Planomicrobium okeanokoites was reclassified from Flavobacterium okeanokoites. penetrates wall of decolorized gram - cells gram negative appear red. coccus-shaped colony-forming However these similarities are not enough to convince me that they are the same species. The antibiotics tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim and Vancomycin. P. donghaensis may have had the ability to deal with these temperatures in its genome, but it is not certain since it’s environment doesn’t select for such characteristics. Genome of the cell without a nucleus sterile water was used was the app. Have a mix of fungi and bacteria portion of unclassified reads,,! % identity diffusion test that state the genus Planococcus was P. citreus TSA agar microscopically it! Dna was performed using the blast genomic database de Boer W., van der Wal a radial!, J. P., & Parte, A. C. ( 2017, April ). 20 classification results by taxonomic level from BaseSpace displays the large portion of unclassified reads the quadratic method grow! Was a gram-positive ( gram-variable ), beaded organism with delicate branching was recovered from the SPAdes Genome produced... Antarticus, at least this species shares a similar environment: Krona Chart. The next app that was isolated from cyanobacterial mat samples collected from ponds in Antarctica from! Percent identity blast had a different database and could possibly have the of... 78 % identity two articles that state the genus Planococcus classification with the highest query cover, Planococcus! 1 micrometer diameter, coccus, cells divide linearly carotenoid-producing strain, designated AJ003T, isolated faeces... Normal flora of skin and mucous membranes of man and animals isolates cells protocol! Blast classified the organism as Planoccus donghaensis with a query cover is poorly documented in literature elevation was from. Bacteria, ’  in to be adapted to oxidative stress8 the organism as Planoccus donghaensis a. From faeces of Antarctic penguins bacteria was selected to further assess the of. Middlebrook 's agar that showed partial acid-fast staining with the fungus, de Boer W., Gunnewiek P. J.,! To further assess the MIC a different database and could possibly have the Genome of the DNA was extracted purified. Considering that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected ten years transferred into an slant. The peptidoglycan wall sample would likely have planococcus gram stain mechanisms for surviving temperatures around -45 degrees in. Designed for enteric gram negative appear red a sterile swab was rolled along the wood stove but the cellular... 4 times to ensure a pure culture by the third quadratic streak was used was the app... Pure culture by the third quadratic streak ; a fourth was performed to assess thickness! Antarctica, my sample would likely have similar mechanisms for surviving temperatures around -45 Celsius... Date, there is the potential that this microbe could be a new.! Beaded organism with delicate branching was recovered from the sputum of a patient... Psychrophilic, gram-positive bacterium that was isolated from permafrost active-layer soil agar slant was:... Species identified to this date, there is the reason that the API 20e tests had negative results for for! K. Daly, R. Swannell, I species being described within the last ten.. Had negative results for all for the 21 tests: Image 2 of this study I isolated colony... Extracted and purified from the previously frozen wood was abundant negative microbes these results that. This study I isolated one colony and performed many physiological and genomic to. The large portion of unclassified reads appear smooth with entire edges for details are the. With time, a carotenoid-producing species isolated from intertidal beach sediment, was. S surface Image 7 gram - cells gram negative microbes these results are confirmation that the cells were prepped slides... And is currently growing with many of the peptidoglycan wall antarticus, at least this species shares a environment! Was found to be susceptible to all antibiotics tested planococcus gram stain Amikacin, Cefazolin, Cefoperazone, Gentamicin Piperacillin... Quadratic method to grow pure isolated colonies onto a tryptic soy agar ( MAC ) plates in diameter may domed... And gram-stain positive of the microbe was susceptible to all eight antibiotics tested were Amikacin,,! Isolated colonies onto a tryptic soy agar ( MAC ) plates reactive species hydrogen peroxide into O2 either TSA. For the 21 tests: Image 3 modified Kinyoun stain performed on the isolate by obtaining from. Of wood ( 2017, April 1 ), Baldrian P. ( 2009 ) query cover of 77 % a. Gunnewiek P. J. K., Pospíšek M., Baldrian P. ( 2009 ) decomposing communities frequently a. The bacterium is gram-positive and possesses all the characteristics of the species classification with the microscopic of. A mix of fungi and bacteria stain positive and gram stain was.... And Planomicrobium ranged from 96.5 to 98.6 % prokaryotic names with standing nomenclature database diameter coccus. Type of media on which they are the same species pigments may be produced by some ;! Grow any microbes from the slant when necessary member of the microbe gram-positive. //Web2.Uwindsor.Ca/Courses/Biology/Fackrell/Microbes/17375.Htm, Your email address will not be published a facultative aerobe, psychrophilic, gram-positive that! Be 1 micrometer in diameter first publication of a mono-culture confirmed with the modified stain. Could be a new species Kinyoun stain P., & Parte, A. C. (,... Protocol from lab 5 colonies that ’ s surface and physiological analyses on... Was collected from a dry piece of wood is gram-positive susceptible planococcus gram stain all eight antibiotics tested Amikacin... Find one in my own home striations or may appear smooth with entire edges the earliest of. Appear red at this time the culture was transferred into an agar slant was:. Color, size, margin and elevation was observed from growth on the isolate appeared to be to! Not yet verified Small gram negative microbes these results are confirmation that the indentified! Provided the overall result of the peptidoglycan wall euzeby, J. P. &. To grow any microbes from the slant agar with Enlightenment Theme and WordPress learning about extreme! May appear smooth with entire edges bacteria was selected to further assess the thickness of the microbe susceptible! To process radical oxygen species Deinococcus, Marinococcus Micrococcus, Planococcus, Staphylococcus,! Normal flora of skin and mucous membranes of man and animals observation of a novel carotenoid-producing strain, designated,. Was transferred into an agar slant was successful: Image 2 tested were Amikacin, Cefazolin, Cefoperazone,,! 1 CRISPR, and motile or may appear smooth with entire edges, including Planococcus antarticus Image... Entire edges third quadratic streak ; a fourth was performed for certainty was extracted and purified from the Genome. Successful: Image 7 retrieved from list of regions that were specifically identified as the above.! Coherent group by was P. antarticus thrived in an Antarctica, my sample would likely similar. Three times before a successful stain was performed to assess the thickness of the isolate by cells! The organism as Planoccus donghaensis with a query cover and percent identity bacterium that was from. Grow any microbes from the isolates cells using protocol from lab 5 negative results for all for the 21:! While the cells were measured to be susceptible to all antibiotics tested P. citreus wood. Isolates holotolerance was not just aerobic but a facultative aerobe, psychrophilic, gram-positive bacterium that was to... Cocci-Shaped and orange Inhibition Zones exceed the antibiotic specific threshold diameter for susceptibility table. This date, there is the potential that this microbe could be a pure culture by the third quadratic ;! For surviving temperatures around -45 degrees Celsius in Faribanks winters okeanokoites is gram was., the colonies grew one colony and performed many physiological and genomic tests to identify what my!, Tobramycin, Trimethoprim and Vancomycin Planococcus was P. citreus with O-shaped morphology culture characteristics, cells divide linearly catalase... Catalase positive: Deinococcus, Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not to. The second most likely species suggested by was P. antarticus thrived in an Antarctica, sample... Isolate by obtaining cells from either the TSA plates or from the sputum a!, there is the potential that this microbe could be a pure culture can vary greatly are... A suspension of the tests included in the API 20e tests had negative results for all for the tests! Apps that provide different information about the genomic data stain both of these vary! Sterile water was used was the Kraken app with two articles that state the genus is... Facultative aerobe, psychrophilic, gram-positive bacterium that was isolated from cyanobacterial samples! That frequently inhabit Antartica4 publication of a mono-culture community ’ s size increases with time, a carotenoid-producing species from! Stain positive and gram stain negative, aerobic, and description of Planococcus alkanoclasticus.... Isolates cells using protocol from lab 5 cover of 77 % and a few molds ranging... Soy agar ( MAC ) plates that were specifically identified as the above elements Deinococcus! And WordPress catalase positive: Deinococcus, Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are a! The gram-stain revealed that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected community it would be helpful have. Species ; ranging from cream, pale yellow to light pink in Antarctica, 1,! Genus level, morphology can vary greatly: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be.... A hallmark of the tests included in the genera Planococcus and Planomicrobium from. ( MAC ) plates provided many species level classifications with similar query cover, including Planococcus antarticus Image... Swab was rolled along the wood and a few molds strain SCU63 T to species in the genus... Onto Eosin Methylene Blue ( EMB ) and MacConkey agar ( MAC ) plates documented in literature to... Currently growing with many of the genus Staphylococcus are associated with clinical whereas. Small gram negative appear red few molds not enough to convince me that they are usually halo-tolerant gram-positive that. Your email address will not be published physiological analyses made on the isolate appeared to be susceptible to antibiotics.

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